Scientists have demonstrated a new technique to non-invasively survey
tigers using their scent sprays, which are detected much more frequently
in the wild than scat--the 'breadcrumb' that researchers have
traditionally used to track the endangered animals. The findings show
that DNA taken from tiger spray is just as good or even better than scat
in identifying individual tigers and their gender, and have the
potential to increase the power of conservation surveys and management.
"Genetic monitoring of tiger source populations is a conservation
priority," said Anthony Caragiulo, a postdoctoral researcher in the
American Museum of Natural History's Sackler Institute for Comparative
Genomics. "The utility of this new method is really impactful because it
will let us dramatically build upon the number of tigers that can be
surveyed and, consequently, increase our understanding of these elusive
animals--hopefully before they are gone."
Despite intense conservation efforts, there are fewer than 3,200 tigers (Panthera tigris) in the wild, living in less than seven percent of their historical range. When a population is confined to small islands of wilderness, as are tigers, there is a higher risk of inbreeding and loss of genetic diversity, leaving the species with weaker young. To combat this, the Museum has been working with the global wild-cat conservation organization Panthera to establish "genetic corridors" that allow tigers to seek new territory for prey and new populations for breeding. "Tracking" individual cats using genetic markers lets researchers map movement within and between populations.
Genetic tracking has traditionally relied on extracting DNA from scat collected in the wild. But in humid, tropical landscapes--like those in Sumatra, where a number of tigers live--scat often degrades before researchers can find it. Scent sprays left by tigers on trees and overhanging leaves degrade less quickly, and can be detected by researchers between two and eight times as frequently as scat. So, to boost the effectiveness of genetic monitoring of tigers in warm regions, the research team questioned whether DNA could be extracted from sprays.
The researchers collected spray samples from three captive tigers in Ontario with cotton swabs that were then stored in tubes of buffer to help preserve the DNA. Tiger spray is a combination of anal gland secretions--said, surprisingly, to have a floral scent like citrus--and urine, which contains DNA in the form of cells from the urethra. The researchers were able to amplify microsatellite loci, which are noncoding DNA sequences with unique numbers of repeated nucleotides providing enough information to "fingerprint" individual tigers, and portions of the sex chromosome to determine whether they are male or female.
"We recently spent weeks looking for tiger scat in the field with very little luck," said Rob Pickles, monitoring specialist for Panthera. "Although this new spray technique wouldn't replace scat studies entirely, we now know that we can use both methods in conjunction to drastically increase our monitoring abilities."
The next step for the researchers is to test the technique in the field, where it also could be used to monitor other scent-spraying animals, like lions.
Despite intense conservation efforts, there are fewer than 3,200 tigers (Panthera tigris) in the wild, living in less than seven percent of their historical range. When a population is confined to small islands of wilderness, as are tigers, there is a higher risk of inbreeding and loss of genetic diversity, leaving the species with weaker young. To combat this, the Museum has been working with the global wild-cat conservation organization Panthera to establish "genetic corridors" that allow tigers to seek new territory for prey and new populations for breeding. "Tracking" individual cats using genetic markers lets researchers map movement within and between populations.
Genetic tracking has traditionally relied on extracting DNA from scat collected in the wild. But in humid, tropical landscapes--like those in Sumatra, where a number of tigers live--scat often degrades before researchers can find it. Scent sprays left by tigers on trees and overhanging leaves degrade less quickly, and can be detected by researchers between two and eight times as frequently as scat. So, to boost the effectiveness of genetic monitoring of tigers in warm regions, the research team questioned whether DNA could be extracted from sprays.
The researchers collected spray samples from three captive tigers in Ontario with cotton swabs that were then stored in tubes of buffer to help preserve the DNA. Tiger spray is a combination of anal gland secretions--said, surprisingly, to have a floral scent like citrus--and urine, which contains DNA in the form of cells from the urethra. The researchers were able to amplify microsatellite loci, which are noncoding DNA sequences with unique numbers of repeated nucleotides providing enough information to "fingerprint" individual tigers, and portions of the sex chromosome to determine whether they are male or female.
"We recently spent weeks looking for tiger scat in the field with very little luck," said Rob Pickles, monitoring specialist for Panthera. "Although this new spray technique wouldn't replace scat studies entirely, we now know that we can use both methods in conjunction to drastically increase our monitoring abilities."
The next step for the researchers is to test the technique in the field, where it also could be used to monitor other scent-spraying animals, like lions.
This Story is taken from Science Daily
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